Part:BBa_K4390043
merR regulated mCherry expression
This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.
This is a level 1 part formed by assembly of the following level 0 parts:
| Promoter | K346002 |
| RBS | B0034 |
| N-O-C part | K180008 |
| Terminator | K4390001 |
Usage and Biology
mCherry is a red fluorescent protein which derived from DsRed of Discosoma (Shaner, N. C. et al., 2004). This protein will generate bright red colour when expressed which is always been used as reporter in research. MerR is a repressor for mercury resistance operons in bacteria. In gram-negative bacteria, the dimeric merR can bind to the promoter of mercury resistance operons which repress the expression. This repression activity is controlled by mercury apperance. When mercury is appeared in the environment, the merR protein will undergo conformational change which will no longer be able to bind to the promoter and release the mercury resistance operons to be expressed (Lund, P. A. & Brown, N. L., 1989).
In this designed part, mCherry will be expressed under regulation of merR, thus mCherry will only be expressed when there are mercury ions present in the environment. In iGEM22_Edinburgh-UHAS_Ghana SELIS design, the expressed mCherry will be used to detect the present of heavy metals, the expression of mCherry will perform a red colour when mercury is present. For further information please refer to SELIS merR evolution construct and SELIS mutated merR evolution construct.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 433
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 433
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 433
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 433
- 1000COMPATIBLE WITH RFC[1000]
References
D'Oelsnitz, S. et al., (2022) Using fungible biosensors to evolve improved alkaloid biosyntheses. Nature chemical biology. 18 (9), 981–989.
Johnson, A. D. et al. (1979) Interactions between DNA-Bound Repressors Govern Regulation by the $\lambda $ Phage Repressor. Proceedings of the National Academy of Sciences - PNAS. 76 (10), 5061–5065.
Shaner, N. C. et al. (2004) Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature biotechnology. 22 (12), 1567–1572.
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